Breast Cancer (Histology Panorama)

© Carolina K. Smith

Breast Cancer (Histology Panorama)

Uploaded: April 12, 2008

Description

Breast Adenocarcinoma. This is a panorama of 9 photos (then cropped) taken with my Nikon D3 and a Leitz Ortholux microscope.
Most cells are in good focus, but some parts will be blurry due to the nature of tissue under the cover slip of the slide.



Exif: F Number: 0, Exposure Bias Value: 0.00, ExposureTime: 8/1000 seconds, Flash: did not fire., ISO: 200, White balance: Manual white balance, FocalLength: 0.00 mm, Model: NIKON D3

Comments

Symona R. Wood April 12, 2008

WOW....how did you get this image? #899494

Colleen Braun April 13, 2008

very interesting... #5780891

Carolina K. Smith April 14, 2008

Thanks for your comments.
To Symona:

If you mean the sample, it is a histology slide from a biological supply company, such as would be viewed in medical schools.
I regularly used such slides in my Pathology rotation.

If you mean 'technique', this has been a hard won process over a couple of years, with things just coming together this year. I place my D3 over a special phototube (which has a photoeyepiece inside the phototube). What is so super about Nikon's D3 is that it has LiveView, so with a USB cable from the camera to my computer, I am able to focus on my my 21 inch monitor!. This is what made a huge difference in my ability to take photomicrographs.

Then I took nine shots over various parts of the slide, keeping some areas in common in order to do a panorama.

I have used AutoPanoPro in the past, but
it didn't do a great job. I did some research and finally upgraded to CS3, and the Photomerge did it perfectly (the matching AND blending, yes, all nine shots. Then I cropped to get rid of the 'circle' look of the microscope objective.

You can see more photomicrographs on my BetterPhoto website (CarolinaSmith.com)

There should be a live link to the left of this message.

Carolina #5785383


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